Plasmid

Part:BBa_K1256013:Design

Designed by: Pei-Hong Chen   Group: iGEM14_Mingdao   (2014-06-10)

pSB1C3-Plac-ccdB-Cm-LacI


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 4045
    Illegal suffix found in sequence at 676
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 4045
    Illegal NheI site found at 221
    Illegal SpeI site found at 677
    Illegal PstI site found at 691
    Illegal NotI site found at 684
    Illegal NotI site found at 4051
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 4045
    Illegal BglII site found at 1966
    Illegal BamHI site found at 533
    Illegal XhoI site found at 1708
    Illegal XhoI site found at 3921
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 4045
    Illegal suffix found in sequence at 677
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 4045
    Illegal XbaI site found at 4060
    Illegal SpeI site found at 677
    Illegal PstI site found at 691
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 442


Design Notes

Source

References